UV Imaging

Many biological molecules of interest naturally fluoresce when excited by shorter- wavelength UV light. This intrinsic fluorescence can be a powerful tool. One important application is the direct fluorescence imaging of aromatic amino acids including tryptophan, tyrosine, and phenylalanine, which are building blocks for proteins. The aromatic rings in these molecules exhibit strong fluorescence excitation…

Splitting Emission Signals

In fluorescence microscopy, there are several options available for viewing multiple colors from a single sample.  The primary benefit of splitting the emission signals using dichroic beamsplitters is the ability to view multiple colors simultaneously rather than sequentially.  This technique of using dichroics to separate emission signals is standard in flow instruments but requires specific…