In fluorescence microscopy, fluorescent molecules are attached to targets of interest to enable quantification, identification and even real-time tracking of activity on a microscopic scale.  The selection of high quality optical filters is essential to getting the sensitivity needed as the the industry demands smaller sample volumes, miniaturization of instrumentation and increased specificity.  These features are important for applications such as the sequencing of DNA, RNA or proteins.

Semrock’s BrightLine® fluorescence filters are brighter, provide better contrast, and have been proven to be far more durable than conventional filters. We stock a complete line of fluorescence filters and sets that are optimized for fluorescence microscopy applications.

We now offer several high-brightness or high-contrast set options in addition to our original balanced sets to allow you to tailor your selection even further to your application.

 

Leading Competitor vs. Semrock Quad-band Filter Set

Comparison of quad band filter sets

Comparisons done under identical imaging conditions using an Olympus BX61WI microscope outfitted with DSU spinning-disk confocal unit and Hamamatsu ORCA-ER monochrome CCD camera. Sample of Rat Kidney Mesangial Cells courtesy of Mike Davidson, Molecular Expressions™, using: Hoechst 33258, Alexa Fluor® 488 – Phalloidin, MitoTracker Red CMXRos, andVimentin (Ms) – Cy5™. Semrock DA/FI/TR/Cy5-4X-A filter set.

 

 

 

 

 

 

 

 

 

 

 

Learn more about high performance microscopy:

Technical Notes

Introduction to Fluorescence Filters

Microscope Cube Assembly Instructions

Orientation of Filters in a Microscope

Multiband Filter Set Terminology

What is Pixel Shift?

Read our feature articles from BioPhotonics

Bringing Superresolution to Fluorescence Microscopy (May/June 2010)

Optical Filters Impact Fluorescence Fidelity (November 2003)

New Optical Filters Improve High-Speed Multicolor Fluorescence Imaging (March 2006)

From the Semrock White Paper Series

Flatness of Dichroic Beamsplitters Affects Focus and Image Quality

Super-resolution Microscopy

Additional Resources

Zeiss Superresolution Microscopy Reference Library

California NanoSystems Institute (CNSI) mini symposium on super resolution optical imaging