Spectral Imaging with VersaChrome®

Download the PDF version   1. Introduction Multicolor fluorescence imaging is ubiquitous in research and clinical applications. When the fluorophores used in such applications have low spectral overlap, standard filter cubes (with fixed spectral edges) are adequate. However, when the crosstalk is high, spectral imaging [1-4] becomes necessary. Spectral imaging systems provide flexibility in wavelength selection.…

Fluorescent Proteins: Theory, Applications, and Best Practices

Download the PDF version   First identified in 1962 in sea creatures, fluorescent proteins have proved versatile and extremely useful, as demonstrated by applications they either enable or significantly benefit. The list includes live cell imaging, multicolor gene expression imaging, and flow cytometry, along with an array of biosensors and optical highlighters. Indeed, fluorescent proteins…

Multimodal NLO Imaging

Download the PDF version   I. Introduction Nonlinear optical (NLO) imaging is a powerful microscopy technique that has found increasing use in the field of biomedical optics. In NLO imaging, ultrafast laser excitation is used to exploit several nonlinear optical effects that can provide high-contrast imaging of biological samples. Common NLO imaging modalities are two- and…