The Physics of Pixel Shift

Download the PDF version   1.  Introduction In multicolored fluorescence microscopy, pixel shift can cause imaging errors, which can lead to erroneous interpretation of biological data [1]. This article highlights some key considerations in the design of optical filter sets with “zero pixel shift” (less than one pixel error) performance. 2. Physics of pixel shift Optical…

Optical Filters for Laser-based Fluorescence Microscopes

Download the PDF version   Introduction Due to many desirable properties such as high brightness, stability, longevity, and narrow spectral bandwidth, lasers have been advantageously replacing conventional broadband light sources for fluorescence imaging applications. Not only have these features of lasers allowed for higher-sensitivity visualization and enhanced throughput in imaging applications, but also several unique…