Splitting Emission Signals

In fluorescence microscopy, there are several options available for viewing multiple colors from a single sample.  The primary benefit of splitting the emission signals using dichroic beamsplitters is the ability to view multiple colors simultaneously rather than sequentially.  This technique of using dichroics to separate emission signals is standard in flow instruments but requires specific…

Ratiometric Imaging – Calcium Imaging

The basis of ratiometric imaging is the sensitivity of certain fluorophores to specific environmental factors.  These environmental factors can include pH, the concentration of certain ions like Ca2+ or the proximity of other fluorophore species (as in FRET).  In the case of calcium imaging, the fluorophore Fura-2 has an absorption spectrum that changes with the…