Multiphoton Microscopy

In multiphoton microscopy, also known as non linear or two-photon microscopy, a sample is illuminated at twice its normal excitation wavelength.  The longer wavelengths are advantageous because they penetrate the sample more deeply for 3D imaging and because they do not damage the sample, allowing increased sample longevity. In order to achieve multiphoton excitation the…

Mercury Line Spectroscopy

Mercury arc lamps continue to be one of the most popular excitation sources for fluorescence microscopy because of the intense brightness they provide.  Semrock offers a line of MaxLamp® mercury line filters that are designed to isloate particular mercury lines while withstanding the intense UV radiation.  These ultrahigh-performance MaxLamp mercury line filters are ideal for…

High-performance Microscopy

In fluorescence microscopy, fluorescent molecules are attached to targets of interest to enable quantification, identification and even real-time tracking of activity on a microscopic scale.  The selection of high quality optical filters is essential to getting the sensitivity needed as the the industry demands smaller sample volumes, miniaturization of instrumentation and increased specificity.  These features…