Flow Cytometry

Flow cytometry is a technique for analyzing a large population of fluorescently labeled cells in a fluid stream.  As the particles pass through a focused light source the amount of light scattered and the emission of a  fluorescence label can be measured.  Flow cytometry is a very important tool in basic research for investigating many…

FRET Fluorescence Resonance Energy Transfer

Fluorescence Resonance Energy Transfer (FRET) is a powerful technique for characterizing distance-dependent interactions on a molecular scale. It is one of the few tools available that is able to measure intermolecular and intramolecular distance interactions both in-vivo and in-vitro.  FRET involves the excitation of a donor fluorophore by incident light within its absorption spectrum.  This…

FISH Fluorescence in Situ Hybridization

Fluorescence in Situ Hybridization is used to visualize defined nucleic acid sequences by hybridization of complementary probe sequences. FISH has many applications, from basic gene mapping to the diagnosis of chromosomal aberrations (Cytogenetics).  Analysis of multicolor FISH images requires isolation of the various signals either with individual filter cubes or an excitation filter wheel in…