BrightLine® Laser Dichroic Beamsplitters

Steeper. Wider. Flatter.

  • λ/10 P-V per inch flatness on 3 mm
  • λ/2 P-V per inch flatness on 1 mm
  • Minimal reflected wavefront distortion for large diameter illumination beams
  • The steepest edges for higher throughput and signal collection
  • Wider reflection bands — into UV for photoactivation and super-resolution techniques
  • Wider transmission regions — into IR to 1200 or 1600 nm

Semrock’s industry leading laser dichroic beamsplitters just got flatter. We’re setting a new standard for super-resolution microscopy with λ/10 P-V per inch flatness on our new 3 mm thick dichroics and improved λ/2 P-V per inch flatness on our improved 1 mm dichroics. Each one comes with our Semrock guaranteed steepest edges, short wavelength reflectivity down to 350 nm, and long wavelength transmission optimized out to 1200 nm or 1600 nm.

tableSuper-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components. In order to address the needs of this market, Semrock has introduced industry leading laser dichroic beamsplitters with λ/10 flatness to minimize the reflected wavefront distortion, thereby maximizing both the signal and the signal-to-noise ratio in super-resolution microscopes.

For setups in which maximizing the switching speed or minimizing the beam shift in transmission is critical, Semrock’s 1 mm thick laser dichroic beamsplitters have been significantly improved to λ/2 flatness (~255 m radius of curvature). These industry leading optics are compatible with popular microscopy filter cubes and improve the performance of laser based confocal and TIRF illumination systems. They are also ideal for reflection of imaging beams in conventional structured-illumination techniques as well as patterned illumination systems for localized photo-activation. These dichroic beamsplitters allow the use of much larger diameter illumination beams, offering researchers and instrument developers more flexibility in system design with no compromise to overall performance.  For more detailed background and information, please review the following tech note.

Flatness Product Code Substrate Thickness Radius of Curvature Application Specification
λ/2 P-V
per inch
Di03-Rxxx-t1-25×36 1.05 mm ~ 255 m Transmission: does not cause significant aberrations to a transmitted beam over the full clear aperture

Reflected Laser Beam: contributes less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror) at the focal plane of a lens after reflecting a laser beam with a diameter up to 10 mm

Reflected Imaging Beam: contributes less than 1.5 x Airy Disk diameter to the RMS spot size of a reflected beam with a diameter up to 16.7 mm

λ/10 P-V
per inch
Di03-Rxxx-t3-25×36 3.0 mm ~ 1275 m Transmission: does not cause significant aberrations to a transmitted beam over the full clear aperture

Reflected Laser Beam: contributes less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror) at the focal plane of a lens after reflecting a laser beam with a diameter up to 22.5 mm

Reflected Imaging Beam: contributes less than 1.5 x Airy Disk diameter to the RMS spot size of a reflected beam with a diameter up to 37.0 mm

 

Super-resolution Microscopy Cubes
Filter sets with guaranteed λ/2 P-V per inch flatness from cube mounted 1mm thick dichroic beamsplitters are available.

 

Laser Dichroic Beamsplitters

Reflected Laser Wavelengths Extended Average Reflection Band (nm) Absolute Reflection Band (nm) Average Transmission Band (nm)

λ/2 Flatness
1 mm Thickness
Filter Part Number 

λ/10 Flatness
3 mm Thickness
Filter Part Number

Availability
375.0 ± 3 nm 405.0 ± 5 nm > 90% 350.0 – 372.0 > 94% 372.0 – 410.0 > 93% 417.4 – 1200 Di03-R405-t1-25×36 Di03-R405-t3-25×36 In Stock
440 +3/-1 nm

442.0 nm

457.9 nm

> 90% 350.0 – 439.0 > 94% 439.0 – 457.9 > 93% 466.1 – 1200 Di03-R442-t1-25×36 Di03-R442-t3-25×36 In Stock
473.0 ± 2 nm 488.0 +3/-2 nm > 90% 350.0 – 471.0 > 94% 471.0 – 491.0 > 93% 499.8 – 1200 Di03-R488-t1-25×36 Di03-R488-t3-25×36 In Stock
505.0 nm

514.5 nm

515.0 nm

> 90% 350.0 – 505.0 > 94% 505.0 – 515.0 > 93% 524.3 – 1200 Di03-R514-t1-25×36 Di03-R514-t3-25×36 In Stock
514.5 nm

532.0 nm

> 90% 350.0 – 514.0 > 94% 514.0 – 532.0 > 93% 541.6 – 1200 Di03-R532-t1-25×36 Di03-R532-t3-25×36 In Stock
561.4 nm

568.2 nm

> 90% 350.0 – 554.0 > 94% 554.0 – 568.2 > 93% 578.4 – 1200 Di03-R561-t1-25×36 Di03-R561-t3-25×36 In Stock
593.5 nm

594.0 -0/+.3 nm

594.1 nm

> 90% 350.0 – 593.5 > 94% 593.5 – 594.3 > 93% 605.0 – 1200 Di03-R594-t1-25×36 Di03-R594-t3-25×36 In Stock
632.8 nm

635.0 +7/-3 nm

647.1 nm

> 90% 350.0 – 632.8 > 94% 632.8 – 641.7 > 93% 658.8 – 1200 Di03-R635-t1-25×36 Di03-R635-t3-25×36 In Stock
785.0 ± 5 nm > 90% 350.0 – 780.0 > 94% 780.0 – 790.0 > 93% 804.3 – 1600 Di03-R785-t1-25×36 Di03-R785-t3-25×36 In Stock
473 ± 2 nm, 488 +3/-2 nm

559 +5/-3, 561.4, 568.2 nm

> 90% 350.0 – 471.0 > 94% 471.0 – 491.0

> 94% 559.0 – 568.2

> 93% 503.3 – 543

> 93% 582.4 – 1200

Di03-R488/561-t1-25×36 Di03-R488/561-t3-25×36 In Stock
375 ± 3, 405 ± 5 nm

473 +2/-0, 488 +3/-2 nm

532.0 nm

632.8, 635 +7/-0, 647.1 nm

> 90% 350.0 – 370.0 > 94% 370.0 – 410.0

> 94% 473.0 – 491.0

> 94% 530.5 – 533.5

> 94% 632.8 – 641.7

> 93% 426 – 462

> 93% 502.5 – 518.5

> 93% 550 – 613

> 93% 663 – 1200

Di03-R405/488/532/635-t1- 25×36 Di03-R405/488/532/635-t3- 25×36 In Stock
375 ± 3, 405 ± 5 nm

473 +2/-0, 488 +3/-2 nm

559 +5/-3, 561.4, 568.2 nm

632.8, 635 +7/-0, 647.1 nm

> 90% 350.0 – 370.0 > 94% 370.0 – 410.0

> 94% 473.0 – 491.0

> 94% 559.0 – 568.2

> 94% 632.8 – 641.7

> 93% 426 – 462

> 93% 502.5 – 544.5

> 93% 582 – 617.5

> 93% 663 – 1200

Di03-R405/488/561/635- t1-25×36 Di03-R405/488/561/635- t3-25×36 In Stock